Hydrolyzed dextran oligosaccharides were separated using HILICpak VN-50 4D, a polymer-based column for HILIC mode, by gradient elution. Gradient elution can detect longer oligosaccharides better than isocratic elution.
Please refer to Hydrolyzed Dextran (6) (VN-50 4D).
Sample : 10µL
Hydrolyzed Dextran 0.5% (in H2O/CH3CN=50/50)
Column : Shodex HILICpak VN-50 4D (4.6mmI.D. x 150mm) Eluent : (A); H2O, (B); CH3CN Linear gradient: 70% (B) to 50% (B) (40min) Flow rate : 1.0mL/min Detector : Corona charged aerosol Column temp. : 40°C