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Advantages of ODP2 HP (4) Ability for Use with Low Salt Concentrations


When 10mM ammonium acetate buffer was used as an eluent, each column showed
a good chromatogram. When the ammonium acetate concentration was lowered to
1mM, both ODS columns showed
tailing peaks caused by interaction between scopolamine and the residual silanol
groups in the column.

Sample
: 5µL
Scopolamine(pKa7.6) 300mg/L

On the other hand, polymer based ODP2
HP-4D
showed no non-specific adsorption of scopolamine to the media even
when the ammonium acetate concentration was lowered. Scopolamine elutes with
a sharp peak. As elution time and peak shapes are not affected even if the salt
concentration in the eluent is lowered,
ODP2 HP
columns are suitable for ESI methods (LC/MS) where salt concentration
in the eluent affects ion suppression of the sample.


Columns : Shodex ODP2 HP-4D (4.6mmID*150mm each)
ODS(A), ODS(B) from other manufacturer (4.6mmID*150mm each)
Eluent : Ammonium acetate buffer(pH7.0)/CH3CN=35/65
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 40°C