Catechin is a kind of polyphenol and is contained a lot in green tea. It is an astringent component of tea. Catechin has been reported to have several bioactivities, like anti-oxidative effect. Six kinds of catechin were analyzed by Silica C18P 4D, a ODS column.
Sample : Catechins
10μg/mL each, 10μL
Column : Shodex Silica C18P 4D (4.6mmI.D. x 150mm) Eluent : (A); 20mM H3PO4 aq. (B); CH3CN Linear gradient: (B%) 20% (0 to 5min), 20 to 40% (5 to 15min), 40% (15 to 20min) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : 30°C