Fructose glucose syrup was analyzed on SUGAR KS-801 according to Japanese Pharmaceutical Excipients 2013 method. It is necessary for the choice of column to satisfy resolution of fructose/glucose of ≥ 1.5 and relative standard deviation (RSD) of six times repeated measurements of ≤ 1.0%. It was confirmed that the all conditions were satisfied when they were analyzed using KS-801.
Sample : 10µL
1. Glucose 60mg/mL
2. Fructose 84mg/mL
Column : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 0.9mL/min Detector : Shodex RI Column temp. : 50°C