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Analysis of Oligopeptides with Acidic and Alkaline Eluent

Oligopeptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography) with acidic and alkaline eluent.
Please refer to Effect of Eluent pH (ODP).

Sample :
1. Glu-Gly-Phe
2. Met-Enkephalin
3. Leu-Enkephalin
4. Leu-Trp-Met

Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent : (1); 0.05% TFA aq./CH3CN=85/15
(2); 50mM Ammonium acetate(pH10.0)/CH3CN=85/15
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : 30°C