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Analysis of Soft Drinks (LC/MS)


Canned soft drink containing coffee and sugar, tea and sugar and orange juice were analyzed using LC/MS. Stable baselines were obtained by using a polymer-based amino column, Asahipak NH2P-50 2D.


Sample : Soft drink
Canned coffee with sugar
1. Sucrose(m/z=378)

Canned tea with sugar
1. Sucrose(m/z=378)

Canned orange juice
1. Fructose(m/z=216)
2. Glucose(m/z=216)


(HPLC)
Instrument : Agilent 1100
Column : Shodex Asahipak NH2P-50 2D (2.0mmID*150mm)
Eluent : H2O/CH3CN=25/75
Flow rate : 0.2mL/min
Column temp. : 40°C
(Post column)
Reagent : CH3CN/CHCl3=50/50
Flow rate : 0.2mL/min
(MS)
Instrument : Agilent 1100MSD
Mass range : 100-500(m/z)
Ionization : APCl
Mode : SIM(M+Cl)
Polarity : Negative
Fragmentor : 20V
Nebulizer : N2(40psi)
Drying gas : N2(10L/min, 350°C)
Corona current : 30micro-A
Vaporizer temp. : 400°C