According to USP 40 method, the assay of tagatose should be used a column packed with L22. It is necessary for the system suitability to satisfy relative standard deviation (RSD) of ≤ 2.0%. It was confirmed to satisfy the system suitability when tagatose was analyzed using SUGAR SC1011.
Sample : 20µL
1. Tagatose 5mg/mL
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : 0.05mg/mL Calcium acetate aq. Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 85°C