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Analysis of Trehalose According to USP Method (KS-801)

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According to USP 40 method, the assay of trehalose and the analysis of related substances in it should be analyzed using a column packed with L58. It is necessary for the system suitability to satisfy resolution (Rs) of trehalose and maltotriose of ≥ 1.5 and relative standard deviation (RSD) of peak area of trehalose ≤ 2.0%. It was confirmed that SUGAR KS-801 satisfies all conditions.

Sample : 0.25% each, 20µL
1. Maltotriose
2. Trehalose
3. Glucose


Column       : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm)
Eluent       : H2O
Flow rate    : 0.4mL/min
Detector     : Shodex RI
Column temp. : 80℃