This shows an example for the detection of androgen (male sex hormone), by an optical rotation detector (ORD) and ODSpak F-411 ( a column for reversed phase chromatography ). Since the peak is negative, 5-androstene-3beta, 17beta-diol is judged to be levorotatory. 5-Androstene-3beta, 17beta-diol is reacted with hydroxysteroid dehydrogenase, and then, 4-androstene-3, 7-dione and testosteronethe are generated. The generation of two components are conceived at 2 min. Since the two peaks are both positive, the components are judged to be dextrorotatory.

Sample :
1. 4-Androstene-3,17-dione
2. Testosterone
3. 5-Androstene-3beta,17beta-diol

 Column : Shodex ODSpak F-411 (4.6mmID*150mm)
 Eluent : CH3OH
 Flow rate : 1.0mL/min
 Detector : ORD
 Column temp. : Room temp.