AFpak APA-894 (an affinity column) which has protein A as the ligand was used for the adsorption and elution of bovine gamma-globulin.
Column : Shodex AFpak APA-894 (8.0mmID*50mm)
Eluent : (A); 0.1M Sodium phosphate buffer(pH4.5)
(B); 0.1M Sodium phosphate buffer(pH3.0)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.