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Saccharides in Bread (KS-801)

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Saccharides are extracted from bread using water and three saccharides, lactose, glucose and fructose are separated using SUGAR KS-801 (a column for saccharides analysis). There is a unknown peak after the peak of fructose. It is recommended to use a guard column for the analysis of actual sample which may contain impurities.


Sample : Bread
1. Lactose
2. Glucose
3. Fructose

 

Columns      : Shodex SUGAR KS-G 6B (6.0mmI.D. x 50mm) + KS-801 (8.0mmI.D. x 300mm)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80°C