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Carbonate and Other Anions (2) (NI-424)

IC NI-424 has high theoretical plate number (more than 5,000). Carbonate and other anions can be separated well with NI-424.

Sample : 50micro-L
1. 40.0mg/L HCO3
2. 4.0mg/L H2PO4
3. 0.3mg/L F
4. 0.5mg/L Cl
5. 1.2mg/L NO2
6. 2.0mg/L Br
7. 2.0mg/L NO3
8. 2.0mg/L SO42-

Column : Shodex IC NI-424 (4.6mmID*100mm)
Eluent : *18mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris
+ 2mM Phenylboronic acid + 0.005mM *2CyDTA(pH4.2)

Flow rate : 1.0mL/min
Detector : Shodex CD
Column temp. : 40°C

*1: The prescribed pH can be obtained by mixing the reagents in the composition described here.
*2: CyDTA: trans-1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic acid