Ion chromatography (cation) column, IC
YS-50 was used to separate mono- and di-valent cations in the presence of albumin. No considerable influence from the presence of albumin was observed. Part of protein (alubumin) may be adsorbed on the column, thus column cleaning using10mM Na2HPO4(pH9-9.5) is recommended after the analysis.
Sample : 10µL
Cation mixture in 7% PEG2000 solution
Column : Shodex IC YS-50 (4.6mmID*125mm)
Eluent : 4mM Methanesulfonic acid aq.
Flow rate : 1.0mL/min
Detector : Non-suppressed conductivity
Column temp. : 40°C