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Cation Analysis in the Presence of Albumin (YS-50)

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Ion chromatography (cation) column, IC
YS-50 was used to separate mono- and di-valent cations in the presence of albumin. No considerable influence from the presence of albumin was observed. Part of protein (alubumin) may be adsorbed on the column, thus column cleaning using10mM Na2HPO4(pH9-9.5) is recommended after the analysis.

Sample : 10µL

Cation mixture in 7% PEG2000 solution

1. Li+

2. Na+

3. NH4+

4. K+

5. Mg2+

6. Ca2+

Column : Shodex IC YS-50 (4.6mmID*125mm)

Eluent : 4mM Methanesulfonic acid aq.

Flow rate : 1.0mL/min

Detector : Non-suppressed conductivity

Column temp. : 40°C