Column Test Conditions for Ligand Exchange and Ion Exclusion Chromatography
We are often asked “What standards should I run to assess the condition of my column?”. Well, here they are for all of our columns. We realize that chromatographers may have columns for years along with several changes of ownership and/or locations. Often, the original COA is lost. We can replace your COA on request. However, you can run the standard samples and asses the column quality even if you no longer have the original COA. Refer to the conditions below. Don’t hesitate to contact us if you need further assistance. Sometimes the column can be regenerated to produce “good as new” quality chromatograms.
| Column type | Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| SC1011
SC1811 (discontinued) SC1011-7F (not sold online) |
(1)1% Glycerol (2)5μL (3)H2O (4)1.0mL/min (5)80°C (6)RI |
| SP0810 | (1)1% Glycerol (2)5μL (3)H2O (4)1.0mL/min (5)80°C (6)RI |
| KS-801 to KS-806
(KS-805-KS-806 are discontinued) |
(1)1% Ethylene glycol(KS-801), 2.5% Ethylene glycol(KS-802), 0.5% Ethylene glycol(KS-803), 0.8% Glucose(KS-804, KS-806), 0.4% Glucose(KS-805) (2)5μL(KS-801, KS-802, KS-804, KS-805, KS-806), 15μL(KS-803) (3)H2O (4)1.0mL/min (5)50°C (6)RI |
| KS-807 (discontinued) | (1)0.5% Glucose (2)5μL (3)H2O (4)1.0mL/min (5)25°C (6)RI |
| SC1211 | (1)1% Glycerol (2)10μL (3)CH3CN/H2O=25/75 (4)1.0mL/min (5)50°C (6)RI |
| DC-613 | (1)2% Sucrose (2)8μL (3)CH3CN/H2O=70/30 (4)0.8mL/min (5)50°C (6)RI |
| SZ5532 | (1)1% Glucose (2)5μL (3)CH3CN/H2O=70/30 (4)1.0mL/min (5)50°C (6)RI |
| MN-431 | (1)0.5% Mannitol (2)10μL (3)H2O (4)0.5mL/min (5)60°C (6)RI |
