IEC DEAE3N-4T is a weak anion exchange
(Diethyaminoethyl) column and fast analysis of proteins and peptides is possible
with non-porous type packing materials. Due to sharp peaks, it is also suitable
to analyze small amount of sample.

Advantages of IEC DEAE3N-4T :

· Rigid particle -> Fast analysis with high flow rate

· No diffusion inside the pores (non-porous type)

· Quick equilibrium against the salt concentration in
eluent –> Sharp peaks

Porous type DEAE-825(
x: diaminotethyl)

Column : Shodex IEC DEAE-825 (8.0mmID x 75mm)
 Eluent : (A); 20mM Piperazine-HCl buffer (pH6.0)
 (B); (A) + 0.5M NaCl (pH6.0)
 Linear gradient (A)?(B),60min
 Flow rate : 1.0mL/min 
 Detector : UV (280nm) 
 Column temp. : Room temp.
 Injection vol. : 100µL 
 

Non-porous type DEAE3N-4T(
x: diaminotethyl)

Column : Shodex IEC DEAE3N-4T (4.6mmID x 35 mm)
 Eluent : (A); 25mM Piperazine-HCl buffer (pH6.0)
 (B); (A) + 0.5M NaCl (pH6.0)
 Linear gradient (A)?(B),10min
 Flow rate : 1.5mL/min 
 Detector : UV (280nm) 
 Column temp. : Room temp.
 Injection vol. : 20µL