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Comparison of NH2P with Silica-based Amino Column (1)

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Polymer-based Asahipak
NH2P-50 4E
column has resolved the problem occurring with silica-based amino columns,
whose performance falls with the time.
1) Conventional analytical
methods used for silica-based amino columns can be applied to the NH2P column.

Eluent —- Mixture of water and acetonitrile which is commonly used for
silica-based amino columns can be also used.
Column temperature —- Can be analyzed at room temperature

2) Excellent quantitative analysis can be performed using NH2P column.

3) Further more, the NH2P column can be washed with alkaline solvent.


Sample :

1. Fructose

2. Glucose

3. Sucrose

4. Maltose


Columns : Shodex Asahipak NH2P-50 4E, Amino column from
another manufacturer (4.6mmID*250mm each)

Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C