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Comparison of Polymer-based Column with Silica-based Column


Asahipak ODP columns are suitable for the analysis of macromolecules such as proteins. A comparison of resolution between the polymer-based (ODP) and the silica-based (ODS) columns shows that the resolution of the former is better.

Sample :
1. Ribonuclease, 2. Cytochrome c, 3. Lysozyme, 4. Chymotrypsinogen A, 5. Ovalbumin


Columns : Shodex Asahipak ODP-50 6D(6.0mmID*150mm), ODS(6.0mmID*150mm)
Eluent : 0.05% TFA aq./CH3CN
Linear gradient: 0 to 30min, 90/10 to 40/60
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : 30°C