BSA and its aggregates were separated with PROTEIN LW-803 which is an aqueous SEC (GFC) column, and a conventional column (KW-803). With KW-803, the tetramer can be detected, however, the pentamer can be detected with LW-803. It is confirmed that LW-803 provided a better separation of the monomer and dimer than KW-803.
Sample : 5μL
BSA 24mg/mL (SIGMA A1900 BSA monomer)
5. BSA monomer (MW 66,000)
Columns : Shodex PROTEIN LW-803 (8.0mmI.D. x 300mm) Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm) Eluent : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : 25°C