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Deoxyribonucleotide (WA-624)

Using AXpak WA-624, a column for weak anion exchange chromatography, four types of deoxyribonucleotide-5′-triphosphates (5′-dNTPs) as raw materials for DNA strand synthesis were separated. The sample was confirmed to contain a small amount of these degradation products, deoxyribonucleotide-5′-diphosphate (5′-dNDPs). WA-624 can separate 5′-dNTPs and 5′-dNDPs, and 5′-dNDPs elutes faster than 5′-dNTPs.


Sample :
5′-dNTPs 0.1 mM each (in H2O), 5 μL
1. 5′-dCTP
2. 5′-dTTP
3. 5′-dATP
4. 5′-dGTP

*The peak a, b, c and d are ５’-dNDPs which are the degradated components from 5′-dNTPs.
a. 5′-dCDP
b. 5′-dTDP
c. 5′-dADP
d. 5′-dGDP

 

Column       : Shodex AXpak WA-624 (6.0 mm I.D. x 150 mm)
Eluent       : 1 M NaH2PO4 aq.
Flow rate    : 1.2 mL/min
Detector     : UV (254 nm)
Column temp. : 60 °C