Using AXpak WA-624, a column for weak anion exchange chromatography, four types of deoxyribonucleotide-5′-triphosphates (5′-dNTPs) as raw materials for DNA strand synthesis were separated. The sample was confirmed to contain a small amount of these degradation products, deoxyribonucleotide-5′-diphosphate (5′-dNDPs). WA-624 can separate 5′-dNTPs and 5′-dNDPs, and 5′-dNDPs elutes faster than 5′-dNTPs.
5′-dNTPs 0.1 mM each (in H2O), 5 μL
*The peak a, b, c and d are ５’-dNDPs which are the degradated components from 5′-dNTPs.
Column : Shodex AXpak WA-624 (6.0 mm I.D. x 150 mm) Eluent : 1 M NaH2PO4 aq. Flow rate : 1.2 mL/min Detector : UV (254 nm) Column temp. : 60 °C