Virus-like particles (VLP) are safe hollow particles with no genetic information, merely the virus’s outer shell. VLPs are widely used in pathology / immunological research such as vaccine preparation, DDS development, and more applications. VLPs are manufactured by microbial expression systems and the like. There are incomplete bodies and aggregates, in addition, to complete spherical bodies in roughly purified products of hepatitis B surface antigen antibody-like particles (HBsAg VLP) that are considered to be VLPs. In this application, a roughly purified product of HBsAg VLP was separated using KW 405-4F, a silica-based aqueous SEC (GFC) column with a multi-angle light scattering detector. When the rms radius of eluted substances was determined, a measurement result reflecting the particle size distribution was obtained.
(Roughly purified product)
Column : Shodex KW405-4F (4.6mmI.D. x 250mm) Eluent : Phosphate buffered saline without magnesium and calcium Flow rate : 0.35mL/min Detector : UV(280nm) (small cell volume) MALS (µDAWN produced by Wyatt Technology Corp.) Column temp. : 25°C