Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)
HPLC method is used for the quantitative analysis of low molecular weight dietary fibers. In this analysis, it is necessary to divide the sample into two fractions, one is hard of digestion fraction composed of saccharides equal or larger than trisaccharide (hard of digestion oligosaccharides + dietary fibers) and another is easy of digestion fraction composed of monosaccharides, disaccharides and sugar alcohols. And, the portion of hard of digestion fraction can be considered as the portion of low molecular weight dietary fibers. Analysis of the low molecule water solubility dietary fiber which used SUGAR KS-802 and Asahipak GS-220 HQ was compared. Using GS-220 HQ, monosaccharides, disaccharides and sugar alcohols elute after the position indicated by the arrow mark and it is easy to divide the two fractions. Therefore, the column can be used for the quantitative analysis of low molecular dietary fibers. Using KS-802, the separation of monosaccharides and disaccharides is better.
Sample : Dietary fiber
Columns : Shodex Asahipak GS-220 HQ (7.6mmID*300mm) x 2 Shodex SUGAR KS-802 (8.0mmID*300mm) x 2
Eluent : H2O H2O
Flow rate : 0.5mL/min 0.3mL/min
Detector : Shodex RI Shodex RI
Column temp. : 60°C 80°C