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DNA Fragments Digested by Hae III (DEAE-420N)


DNA fragments digested by Hae III were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).


Sample : phi-X-174-RF DNA fragment

No.
base pairs
MW (x 105daltons)
1.
1353
8.79
2.
1078
7.01
3.
872
5.67
4.
603
3.92
5.
310
2.02
6B.
281
1.83
6A.
271
1.76
7.
234
1.52
8.
194
1.26
9.
118
0.77
10.
72
0.47



Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Phosphate buffer(pH5.2)
(B); (A) + 1M NaCl
Linear gradient: 0min to 20min, 75% (B) to 100% (B)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30°C