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Durability against Acidic Solvents


Since Asahipak NH2P-50 column is chemically stable,
the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedure:

(1) Testing (63hrs)
Eluent : 0.1M H2SO4 (pH2)
Flow rate : 0.1mL/min
Column temp. : Room temp.

(2) Replacement (1hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.

(3) Equilibrium (1hr)
Eluent : 0.1M NaOH
Flow rate : 0.5mL/min
Column temp. : Room temp.

Comparison between before and after testing:

Sample Before testing After testing
Elution volume
(mL)
Plate number Elution volume
(mL)
Plate number
1.Fructose 6.73 9600 6.70 8400
2.Glucose 8.59 7900 8.58 8500
3.Sucrose 11.88 9600 11.93 9300
4.Maltose 14.24 8500 13.90 8600



Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C