Effect of Acetonitorile Concentration on Elution Volume
Monovalent and divalent cation standards were analyzed using IC YK-421. The effect of the acetonitorile concentration in 3mM nitric acid on elution volume of cations is shown. By increasing the acetonitorile concentration, the cations are eluted faster. Especially, the elution volume of divalent cations are eluted faster markedly. When the acetonitorile concentration of acetonitrile is higher than 50%, to the contrary, cations are eluted slower a little by increasing acetonitorile concentration.