Ribosomal RNA was analyzed using Asahipak GS-620 7G (a column for GFC separation). For general HPLC analysis, a high flow rate of 0.5-1.0mL/min or over is generally used. Whereas, for the GFC analysis of high molecular weight components, it is recommended that the analysis be made at a flow rate as low as possible, e.g. 0.1mL/min to prevent molecular chains from being cut off and to permit a high resolution. GS-620 7G is an old type of GS-620 HQ.