When Asahipak NH2P-50 is used, it is recommended to use
line filter, NH2P-LF with it. Figure (A) and (B) show the
effect of NH2P-LF. Figure (A) shows that the column resolution is same for about
135 hours, however, figure (B) shows that the column resolution decreases just
after it is used.
Regarding figures (a), (b) and (c), refer to
Effect of Line Filter NH2P-LF (1).
Sample : 0.2% each, 5micro-L
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25°C