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Effect of pH on Retention Time (NI-424)

Effects of pH on Retention Time (NI-424)

Effects of eluent pH on the elution of each anion using IC NI-424
(an anion analysis column for non-suppressor method) are shown. When the pH of the eluent is increased, the retention time for the anions is decreased.

Sample : 20?L
H2PO4 10mg/L
F 10mg/L
Cl 10mg/L
NO2 10mg/L
Br 10mg/L
NO3 10mg/L
I 20mg/L
SO42- 10mg/L
S2O32- 50mg/L
SCN 50mg/L
ClO4 50mg/L

Column : Shodex IC NI-424 (4.6mmI.D. x 100mm)
Eluent : 8mM 4-Hydroxybenzoic acid + 2mM Phenylboronic acid
+ 0.005mM *CyDTA(pH adjusted with Bis-Tris)
Flow rate : 1.0mL/min
Detector : Non-suppressed conductivity
Column temp. : 40°C

*CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid