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Effect of Sample Load on Column Efficiency (4) (NI-424)

The column efficiency of IC NI-424 does not decline with an injection weight of up to 500 ng for chloride ion and of 5,000ng for sulphate ion. Concerning injection volume, an injection volume of up to 500micro-L does not cause any noticeable decline in the column efficiency at a concentration of 1 mg/L for both chloride and sulphate ions.

Sample :
1. Cl
2. SO42-

Column : Shodex IC NI-424 (4.6mmID*100mm)
Eluent : *18mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris
+ 2mM Phenylboronic acid + 0.005mM *2CyDTA(pH4.2)

Flow rate : 1.0mL/min
Detector : Shodex CD
Column temp. : 40°C

*1 The prescribed pH can be obtained by mixing the reagents in the composition described here.
*2 CyDTA: trans-1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic acid