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Effects of Column Temperature on Elution Volume (1) (KC-811)

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Effects of column temperature on elution volume when RSpak KC-811 is used is shown.

Substance Elution Volume (mL)
30°C 40°C 50°C 60°C 70°C 80°C
Oxalic acid
9.66
9.63
9.62
9.56
9.51
9.47
Maleic acid
10.74
10.65
10.51
10.53
10.47
10.41
Phosphoric acid
11.01
11.14
11.27
11.41
11.53
11.70
α-Ketoglutaric acid
11.08
10.95
10.87
10.77
10.70
10.64
2-Ketogluconic acid
11.56
11.52
11.52
11.47
11.44
11.42
Glucuronic acid
11.67
11.69
11.71
11.69
11.68
*
Pyruvic acid
11.91
11.82
11.76
11.72
11.70
11.71
Citric acid
11.93
11.81
11.71
11.57
11.46
11.36
Oxaloacetic acid
12.00
11.86
11.77
11.76
11.73
11.70
Isocitric acid
12.14
11.99
11.88
11.72
11.60
11.48
Tartaric acid
12.36
12.23
12.14
11.99
11.90
11.81
Galacturonic acid
12.48
12.44
12.41
12.31
12.26
12.20
Gluconic acid
12.79
12.78
12.78
12.76
12.90
12.91
Malonic acid
13.26
13.09
12.96
12.81
12.71
12.61
trans-Aconitic acid
13.99
13.46
13.11
12.74
12.47
12.24
Glyoxylic acid
13.30
13.27
13.26
13.19
13.14
13.09
Malic acid
13.69
13.51
13.37
13.19
13.04
12.91
Ascorbic acid
14.13
13.99
13.88
13.72
13.59
*
α-Hydroxyglutaric acid
14.58
14.25
14.05
13.83
13.98
13.91
Succinic acid
16.53
16.11
15.79
15.40
15.10
14.82
Glycolic acid
16.55
16.39
16.26
16.06
15.89
15.74
Lactic acid
16.83
16.75
16.67
16.51
16.37
16.21
2-Hydroxyisobutyric acid
17.02
17.06
17.05
16.94
16.82
16.66
β-Hydroxypropionic acid
17.53
17.34
17.18
16.96
16.76
16.57
Itaconic acid
17.83
17.26
16.80
16.29
15.87
15.48
Formic acid
18.23
18.03
17.86
17.64
17.46
17.28
Fumaric acid
19.23
18.02
17.16
16.39
15.79
15.30
Acetic acid
19.92
19.66
19.41
19.11
18.82
18.55
Uric acid
20.71
19.28
18.31
17.40
16.75
16.22
Levulinic acid
22.06
21.07
20.27
19.53
18.87
18.27
Pyroglutamic acid
22.91
21.80
20.99
20.33
19.77
19.29
Adipic acid
23.10
21.41
20.17
19.03
18.11
17.34
Propionic acid
23.53
23.02
22.54
22.01
21.49
20.69
Mesaconic acid
24.02
21.93
20.47
19.15
18.14
17.31
CO32-, HCO3
24.29
24.48
24.50
24.51
24.35
24.15
Isobutyric acid
27.10
26.35
25.59
24.77
23.95
23.14
n-Butyric acid
29.76
28.66
27.61
26.53
25.49
24.48
Isovaleric acid
35.65
33.92
32.28
30.60
29.00
27.46
n-Valeric acid
45.05
41.77
38.89
36.12
33.60
31.27

* : cannot be detected

Columns   : Shodex RSpak KC-811 (8.0mmI.D. x 300mm) x 2
Eluent    : 3mM HClO4 aq.
Flow rate : 1.0mL/min