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Elution Volume of Saccharides and Organic Acids


Elution volume of saccharides and organic acids using SUGAR SH1011 is summeraized. When the concentration of sulfuric acid in the eluent is changed, the elution volume of organic acids changes in some degree though that of saccharides changes very little. Optimal concentration of sulfuric acid should be selected according to the sample to be analyzed.

Saccharides
Sugar alcohols
(a)
5mM
(b)
20mM
(b)/(a)
Stachyose 8.59min 8.66min 100.85%
Isomaltotriose 8.85 8.90 100.59
Panose 8.94 8.98 100.48
Maltotriose 9.03 9.09 100.65
Gentiobiose 9.47 9.51 100.42
Isomaltose 9.63 9.69 100.64
Melibiose 9.77 9.82 100.51
Kojibiose 9.79 9.83 100.44
Maltose 9.80 9.86 100.60
Trehalose 9.82 9.87 100.53
Nigerose 9.87 9.87 99.99
Palatinose 9.96 9.75 97.91
Palatinit 9.96 10.04 100.77
Trehalulose 9.97 10.02 100.51
Lactose 9.99 10.05 100.55
Xylobiose 10.09 10.15 100.52
Maltitol 10.19 10.27 100.78
Lactitol 10.45 10.54 100.84
Glucose 11.32 11.37 100.47
Sorbose 11.54 11.58 100.32
myo-Inositol 11.69 11.75 100.47
D-Mannose 11.85 11.90 100.44
D(+)-Galactose 11.89 11.94 100.44
D(+)-Xylose 11.92 11.97 100.43
D(-)-Fructose 12.01 12.05 100.37
Mannitol 12.26 12.34 100.63
D(-)-Sorbitol 12.42 12.47 100.39
D(+)-Rhamnose 12.46 12.51 100.41
D(+)-Arabinose 12.74 12.79 100.40
D(-)-Ribose 13.01 13.04 100.22
D-Arabitol 13.07 13.14 100.57
Xylitol 13.25 13.33 100.61
D(+)-Fucose 13.35 13.40 100.40
meso-Erythritol 13.81 13.89 100.60
N-Acetyl-alpha-D-
glucosamine
14.02 14.05 100.21
N-Acetyl-alpha-D-
galactosamine
15.47 15.49 100.12
Organic Acids (a)
5mM
(b)
20mM
(b)/(a)
Oxalic acid 8.46min 9.38min 110.90%
D-Glucuronic acid 10.24 10.39 101.46
Citric acid 10.25 10.48 102.26
alpha-Ketoglutaric acid 10.27 11.47 111.66
Maleic acid 10.28 11.77 114.46
N-Acetylneuramic acid 10.53 10.94 103.92
Tartaric acid 10.67 11.06 103.65
Pyruvic acid 11.48 12.79 111.36
Malic acid 11.74 11.93 101.63
Malonic acid 11.95 12.53 104.84
trans-Aconitic acid 12.01 12.51 104.10
Succinic acid 13.78 13.83 100.39
DL-Lactic acid 14.73 14.86 100.85
Formic acid 15.70 15.82 100.78
Acetic acid 16.90 16.93 100.18
Adipic acid 17.32 17.42 100.59
Mesaconic acid 18.28 19.02 104.07
L-Pyroglutamic acid 19.00 19.53 102.77
Propionic acid 19.45 19.54 100.40

Alcohol (a)
5mM
(b)
20mM
(b)/(a)
Ethanol 23.43min 23.52min 100.38%



Column : Shodex SUGAR SH1011 (8.0mmID*300mm)
Eluent : 5mM or 20mM H2SO4 aq.
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 60°C