Fatty acids were analyzed using ODSpak F-411 (a column for reversed phase chromatography).

Sample :
1. EPA, Eicosapentaenoic acid)
2. DHA, Docosahexaenoic acid)
3. Arachidonic acid
4. Linoleic acid

 Column : Shodex ODSpak F-411 (4.6mmID*150mm)
 Eluent : H2O/CH3CN=20/80
 Flow rate : 0.7mL/min
 Detector : Shodex RI
 Column temp. : 30°C