ET-RP1 4D is polymer base column for high temperature analysis.
Figure shows comparison of reduced plate height and analysis time between 40°C and 150°C.
Smaller value of reduced plate height means better column resolution. The reduced plate height
of acetophenone and benzene decreased at the 150°C.
Mobile phase viscosity decreases at higher temperature and thus enhanced diffusion rate
between the eluent and the polymeric stationary phase. Then resolution is improved.

Sample :

1. Uracil

2. Pyridine

3. Acetophenone

4. Benzene

(notes) Eluent was introduced to column after preheating and introduced to detector after cooling.

*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)

Coutesy of Research Institude for Chromatography bvba