ET-RP1 4D is polymer base column for high temperature analysis.
Figure shows comparison of reduced plate height and analysis time between 40°C and 150°C.
Smaller value of reduced plate height means better column resolution. The reduced plate height
of acetophenone and benzene decreased at the 150°C.
Mobile phase viscosity decreases at higher temperature and thus enhanced diffusion rate
between the eluent and the polymeric stationary phase. Then resolution is improved.
(notes) Eluent was introduced to column after preheating and introduced to detector after cooling.
|Column||: Shodex ET-RP1 4D (4.6mmID*150mm)||: Shodex ET-RP1 4D (4.6mmID*150mm)|
|Eluent||: H2O/CH3CN=50/50||: H2O/CH3CN=75/25|
|Flow rate||: 0.5mL/min||: 2.4mL/min|
|Detector||: Diode Alley (210nm)||: Diode Alley (210nm)|
|Column temp.||: 40°C||: 150°C|
*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)
Coutesy of Research Institude for Chromatography bvba