is polymer base column for high temperature analysis.
Figure shows comparison chromatograms between 40°C and 80°C.
Mobile phase viscosity decreased at high temperature analysis, higher flow rate can be applied.
And suitable flow rate range is shifted to higher at high temperature.
ET-RP1 4D is good column for fast analysis with high temperature analysis.
Sample : 5micro-L, 10mg/L each
* Eluent was introduced to column after preheating and introduced to detector after cooling.
* Preheat eluent when high flow rate (more than 1mL/min) is applied.
Column : Shodex ET-RP1 4D (4.6mmID*150mm)
Eluent : CH3CN/H2O=40/60
Detector : UV(210nm)
*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)