Some metabolites in urine have been added to the health check as special items in Japan recently. From the items, hippuric acid, methylhippuric acid (o-, m-, p-) and mandelic acid which are metabolites of toluene, xylene and styrene and creatinine were analyzed using ODSpak F-411 ( a column for reversed phase chromatography) within 10 minutes.

Sample :
1. Mandelic acid
2. Hippuric acid
3. o-Methylhippuric acid
4. p-Methylhippuric acid
5. m-Methylhippuric acid
6. Creatinine

 Column : Shodex ODSpak F-411 (4.6mmID*150mm)
 Eluent : [CH3CN/Buffer=12/88]+4.5mM sodium dodecyl sulfate
 (Buffer: 20mM H3PO4+20mM beta-Cyclodextrin (pH2.5))
 Flow rate : 1.0mL/min
 Detector : UV(225nm)
 Colomn temp.: 50°C