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Hydrophobicity of Columns


The ratio of retention time of Butyl alcohol and Ethylene glycol shows hydrophobicity of column.This figure shows the result that we measured the strength of the hydrophobicity of various columns with water as eluent.


Sample:
Butyl alcohol
Ethylene glycol


Columns : Shodex OHpak SB-802 HQ, SB-802.5 HQ,
SB-803 HQ, SB-804 HQ (8.0mmID*300mm each)
Shodex Asahipak GS-220 HQ, GS-320 HQ,
GS-520 HQ, GF-310 HQ, GF-510 HQ (7.6mmID*300mm each)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 30°C