Iodide is an essential element in the human body to make thyroid hormone. Iodide was analyzed using HILICpak VT-50 2D, a polymer-based HILIC mode column, with LC/MS detection. High acetonitrile concentration in the eluent is suitable for the analysis with higher sensitivity and selectivity than ion chromatography.
Sample : 5μL
1. I–, Iodide 1ng/mL (in H2O)
Column : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm) Eluent : 50mM HCOONH4 aq./CH3CN=20/80 Flow rate : 0.3mL/min Detector : ESI-MS (SIM Negative) Column temp. : 40°C