A method was optimized for the simultaneous analysis of anionic and non-ionic surfactants using an Aqueous/Organic (multi-solvent use) SEC column, Asahipak GF-210 HQ. Polyvinyl alcohol used for GF-210 HQ packed gel is slightly negatively charged. By using mobile phase without salt retains this negatively charged surface characteristic; this results in the fast elution of anionic surfactant by ion exclusion effect, and thus separates it from non-ionic surfactant. Meanwhile, addition of salt to the mobile phase suppresses the ion exclusion effect and retains the anionic surfactant, thus it is not separated from the non-ionic surfactant.
Sample : 50μL
1. Sodium p-octylbenzenesulfonate 100mg/L
2. Triton X-114 (Polyoxyethylene(8) octylphenyl ether) 1000mg/L
Column : Shodex Asahipak GF-210 HQ (7.5mmI.D. x 300mm) Flow rate : 0.8mL/min Detector : UV(254nm) Column temp. : 40°C