Gelatin contains alpha, beta and gamma chains, and the ratio of their content is said to have a significant effect on the performance of gelatin. The Asahipak GS-620 HQ column is suitable for general analysis of the components of gelatin and Asahipak GS-620 7G-P is especially suitable for analysis of the components of gelatin by the PAGI method(test method of gelatin for photo film in Japan). When using a Asahipak GS-620 7G-P, the column should be pretreated in accordance with “Precautions in Analyzing Gelatin” attached to the operation manual so as to obtain reproducible data.
Sample : Gelatin
Columns : Shodex Asahipak GS-620 7G-P (7.6mmID*500mm) x 2
Eluent : 0.2M NaH2PO4(pH6.8)
Flow rate : 0.57mL/min
Detector : UV(220nm)
Column temp. : 50°C
Courtesy of Dr. Ohno, Chiba University