Monosaccharides and disaccharides were analyzed by HILICpak VG-50 4E, a polymer-based amino column. The recovery ratio of reducing sugars by VG-50 is high, so the peak of galactose can be detected higher than by the former amino columns. VG-50 is suitable for high sensitive analysis.
Sample : 0.05% each, 10μL
Column : Shodex HILICpak VG-50 4E (4.6mmI.D. x 250mm) Eluent : CH3CN/CH3OH/H2O=85/5/10 Flow rate : 1.0mL/min Detector : Corona charged aerosol Column temp. : 60°C