Deoxynivalenol(DON) and Nivalenol(NIV) in grains were analyzed with Silica C18M 4E after the sample pretreatment.
Sample : 20μL
(A) Standard solution (The amount of DON and NIV are 10ng each.)
(B) Sample solution of Wheat spiked with DON and NIV at 1μg/mL each
Column : Shodex Silica C18M 4E (4.6mmI.D. x 250mm) Eluent : H2O/CH3OH/CH3CN=90/5/5 Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : 40°C