Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography). pH of the eluent is heightened to 5.5.
Please compare with the cases of pH4.3 and pH5.0

Sample :
1. 5’CDP
2. 5’UDP
3. 5’ADP
4. 5’GDP

 Column : Shodex AXpak WA-624 (6.0mmID*150mm)
 Eluent : 0.7M CH3COONH4 buffer(pH5.5)
 Flow rate : 1.0mL/min
 Detector : UV(260nm)
 Column temp. : 60°C