Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography). pH of the eluent is lowered to 4.3. Retention becomes stronger and separation becomes better. Please compare with the cases of pH5.0 and pH5.5

Sample :
1. 5’CMP
2. 5’UMP
3. 5’TMP
4. 5’AMP
5. 5’GMP
6. 3’AMP
7. 3’GMP
8. 5’CDP
9. 5’UTP

 Column : Shodex AXpak WA-624 (6.0mmID*150mm)
 Eluent : 0.7M CH3COONH4 buffer(pH4.3)
 Flow rate : 1.0mL/min
 Detector : UV(260nm)
 Column temp. : 60°C