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Oligopeptides (1) (ODP-50 6D)

 

Oligopeptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).


Sample :
1. Val-Glu-Glu-Ala-Glu
2. Gly-Gly-Phe
3. Met-Enkephalin
4. Bradykinin
5. Angiotensin II
6. Angiotensin III
7. Phe-Phe-Phe

 Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
 Eluent : (1): (A); 0.1M NaClO4 + 0.1% Sodium phosphate(pH2)
 (B); CH3CN
 Linear gradient: 0min to 60min, (A)/(B)=90/10 to 60/40
 (2): (A); 0.1M NaClO4 + 0.1% Sodium phosphate(pH9)
 (B); CH3CN
 Linear gradient: 0min to 60min, (A)/(B)=90/10 to 60/40
 Flow rate : 1.0mL/min
 Detector : UV(220nm)
 Column temp. : 30°C