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Orange Juice (SC1011)

 

Saccharides in orange juice were analyzed using SUGAR SC1011 (a column for saccharides analysis). For the pretreatment of juice, first, the sample was filtrated 2 times using a 0.45 micron disposal filter. Then, it was treated with a mini-column packed with anion ion exchanger. And it was filtered again. In front of glucose and fructose peaks, a large peak was observed and it was considered to be oligosaccharides.


Sample : Mandarin orange juice, 5micro-L
1. Sucrose
2. Glucose
3. Fructose

 Column : Shodex SUGAR SC1011 (8.0mmID*300mm)
 Eluent : H2O
 Flow rate : 1.0mL/min
 Detector : Shodex RI
 Column temp. : 80°C