The ratio of deproteinization with MSpak GF-4A (a column for Column switching) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, GF-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis. (GF-2A (2.0mmI.D. x 10mm) is a custom-made column of GF-4A.)
|Ratio of Deproteinization|
Pretreatment Eluent : 10mM CH3COONH4(pH7.0) Flow rate : 0.5mL/min Column temp. : Room temp. Switching time : 5min Analysis Column : Shodex PROTEIN *KW-604S (6.0mmI.D. x 50mm) *KW-604S is phase-out product. Eluent : 0.1% TFA in (H2O/CH3CN=50/50) Flow rate : 0.5mL/min Detector : UV(280nm) Column temp. : Room temp.