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Saccharide Analysis Using Semi-micro Column

 

High sensitive analysis of saccharides was performed using Asahipak NH2P-50 2D.


Sample : 1.0% each, 20micro-L
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose

 Columns : Shodex Asahipak NH2P-50 2D Shodex Asahipak NH2P-50 4D 
 (2mmID*150mm) (4.6mmID*150mm)
 Eluent : CH3CN/H2O=75/25 CH3CN/H2O=75/25
 Flow rate : 0.2mL/min 1.06mL/min
 Detector : Shodex RI(small cell volume) Shodex RI(conventional type)
 Column temp. : 25°C 25°C