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Saccharides in Powdered Milk (NH2P-50 4E)

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High sensivity analysis of saccharides in powdered milk was performed using Asahipak NH2P-50 4E (a column for saccharides analysis) and an electrochmical detection system.

Sample : powdered Milk
1. Lactulose
2. Lactose

 Instrument : Agilent 1050 system
 Reagent : 0.6M LiOH 0.8mL/min
 Mode : pulse
 Working electrode : Gold
 Potential : Pot 1=0.65V, Pot 2=-0.95V, Pot=0.15V

 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : (A); CH3CN/H2O=80/20
 (B); H2O
 0min to 40min, 5% (B) to 40% (B)
 40min to 45min, 40% (B)
 Flow rate : 1.0mL/min
 Detector : Agilent 1049A Electrochemical
 Column temp. : 40°C