Six kinds of protien standard were analyzed using IEC CM-825 ( a column for weak cation exchange chromatography ) and IEC SP-825 ( a column for strong cation exchange chromatography ).
1. Myoglobin, 2. Trypsinogen, 3. Ribonuclease A, 4. alpha Chymotrypsinogen A, 5. Cytochrome c, 6. Lysozyme
Columns : (Left) Shodex IEC CM-825 (8.0mmID*75mm)
(Right) Shodex IEC SP-825 (8.0mmID*75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH7.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 60min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.