Since corona charged aerosol detector (CAD) measures components of elute as well as the analyte, anything elute from column influences the baseline largely. The polymer-based amino column, Asahipak NH2P-50 4E, has very small “bleeding” and thus provides stable baseline without much noise compared to silica-based columns.
Sample : 40µg/mL each, 5µL
Columns : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm) Silica based amino column from other manufacturer (4.6mmID*250mm) Silica based amide column from other manufacturer (4.6mmID*150mm) Eluent : H2O/CH3CN=20/80 Flow rate : 1.0mL/min Detector : Corona charged aerosol Column temp. : 30°C (Amino column), 80°C (Amide column)