Synthetic food colorants Red 2, Red 102, as well as azorubine and patent blue V are commonly added to red wine to deepen its red color. In this application, these 4 synthetics food colorants were analyzed at high temperature with the polymer-based reversed phase chromatography cplumn ET-RP1 3D. The high-temperature analysis was completed in about 10 min, compared to a usual analysis time of over 40 min.
Column : Shodex ET-RP1 3D (3.0mmID*150mm)
Eluent : 6mM Tetrabutylammonium bromide in (25mM Ammonium acetate aq./CH3CN=70/30)
Flow rate : 0.5mL/min
Detector : UV(254nm)
Column temp. : 70°C